期刊论文

Preparation of Cellulose-Alginate-Chitosan Complex Films for Tissue Engineering Skin and Their Characteristics

中文

医学分子生物学杂志

1672-8009

2014

3

165-168

10.3870/j.issna.1672-8009.2014.03.008

(81102409):国家自然科学基金 (2014 GK3082):湖南省科学技术厅课题 (10 C1179):湖南省教育厅项目 (No．2010 XQD37):南华大学抗肿瘤药物创新团队，南华大学博士启动基金 (81102409):This work was supported by grants from the National Natural Science Fundation of China (2014GK3082):Research Grant of De-partment of Science and Technology Project of Hunan Province (10C1179):Scientific Research Fund of Hunan Provincial Education Department (2010 XQD37):the Innovation Team of Antitumor Drug and the PhD Start-up Fundation of University of South China

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目的：为了筛选出适合用于组织工程皮肤的膜支架材料，采用不同来源的纤维素（木、棉、竹），与壳聚糖以及海藻酸钠等多糖为原料制备了多种复合膜，并表征了其理化性能。方法分别配制木、棉、竹纤维素溶液，海藻酸钠溶液和壳聚糖溶液，分别采用木、棉、竹3种纤维素溶液作为基底膜，然后依次流延一层壳聚糖膜和一层海藻酸钠膜，从而制得3种不同来源的纤维素-壳聚糖-海藻酸盐复合膜。应用荧光倒置显微镜观察3种复合膜的微观形态结构。将复合膜浸泡在生理盐水中，观察复合膜的形态变化来判定复合膜的盐水耐受性。运用接触角分析仪观察并测定不同复合膜与水的接触角的大小及其随时间的变化。结果在荧...

Objective To prepare a variety of complex films with alginate , chitosan , and cel-lulose of different origins in order to identify the optimal film for tissue engineering skin .Methods The sodium alginate , chitosan and cellulose ( cotton, wood and bamboo ) solutions were prepared re-spectively.The complex films were prepared by using the cellulose (cotton, wood and bamboo) film as the basement , and then casting one layer of sodium alginate and another layer of chitosan on the basement in turn.Fluorescence inverted microscopy was used to observe the microstructures of the three kinds of comp...