Objective To construct ds cDNA of HL-60 cells in yeast two-hybrid sys- tem. Methods With the SMART technology supplied by CLONTECH Company, the total RNA of HL-60 ceils was extracted and reversed to form the first cDNA, and ds cDNA was then synthesized through LD PCR. The ds cDNA was co-transformed with pGADT7-Rec into AH109 strain. Results The ds cDNA of HL-60 cells was successfully constructed in yeast two-hybrid system. AD co-transfor- mation efficacy reached up to 8×10^4 cfu/3μg pGADT7-Rec. Conclusion The ds cDNA of HL-60 cells can act as...
