期刊论文

中文

国际麻醉学与复苏杂志

1673-4378

2009

30

4

332-334,372

10.3760/cma.j.issn.1673-4378.2009.04.014

江苏省级高校重点实验室开放课题资助项目（KJS06002）；

本校为其他机构

目的 观察鞘内注射(intrathecal,i·t)加巴喷丁和吗啡对切口痛大鼠脊髓氨基酸类神经递质的影响.方法 选择鞘内置管成功的雄性SD大鼠40只,随机分为5组,每组8只,分别为假手术组,对照组,加巴喷丁50μg组,吗啡2.5μg组,加巴喷丁50μg加吗啡2.5 μg组.按Yaksh法鞘内置管;按Brennan法制作大鼠足底切口疼痛模型;用机械缩爪反射阈值(mechanical withdrawal threshold,MWT)和热缩爪潜伏期(thermal withdrawal latency,TWL)来确定疼痛行为学变化;应用高效液相色谱法(HPLC)测定脊髓氨基酸类神经递质如谷氨酸(glutamate,Glu)、天门冬氨酸(aspartate,Asp)、甘氨酸(glycine,Gly)、γ-氨基丁酸(γ-aminobutvric...

Objective To study the effects of intrathecally administering gabapentin and/or morphine on the amino acid neu- rotransmitters in spinal cord of rat incision pain models. Methods Forty male SD rats were randomly divided into five groups （n=8） and were intrathecally inserted catheter. Paw incision models were made 5d later. Gabapentin （50 μg, group Ⅲ ） or morphine（2.5 μg, group IV ） or both （group V ） were intrathecally injected 30 min before incision. Another two groups were regarded as shame （group I ） or control （group Ⅱ ）. Pa...