Objective To screen and identify the interactive proteins of CLN8P and provide clues for the studies on CLN8 function and pathogenesis of NCLs. Methods The Human Fetal Brain cDNA library was screened with with pLexA-CLN8 as bait plasmid by yeast-two hybrid system, and Leu^+ and LacZ^+ positive yeast clones were obtained, which were cotransformed to the EGY48 (p8op-LacZ) along with pLexA- CLN8 plasmid one to one by yeast simultaneous cotransformation so as to identify the protein interactions again. The inserted fragments of identified posit...