期刊论文

Liu, GX

英文

中华医学杂志(英文版)

0366-6999

2002

115

5

750-752

10.3760/j.issn:0366-6999.2002.05.023

ThisprojectwassupportedbytheNationalMajorBasicProgram（G2 0 0 0 5 690 5 ）； NationalNaturalScienceFoundationofChina（3 9470 80 9）；

本校为第一且通讯机构

Objective. To study the features of vascular smooth muscle cell (VSMC) proliferation induced by endothelin-1 (ET-1). Methods. VSMCs of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats were cultured and treated with ET-1. Basic fibroblast growth factor (bFGF) gene expression was measured using both Northern blot and an enzyme-linked immunoassay. Results. ET-1 resulted in an increase in bFGF transcripts at 8-24 h; bFGF levels were significantly higher in VSMCs treated with ET-1 than in those not treated. However, VSMCs growth responses...

目的研究自发性高血压大鼠平滑肌细胞增殖特性.方法采用细胞培养、核酸杂交、酶联免疫法及反义核酸技术检测内皮素对自发性高血压大鼠和Wi star-kyoto大鼠的血管平滑肌细胞增殖、碱性成纤维细胞生长因子表达的影响,并进行对比分析.结果内皮素-1诱导了血管平滑肌细胞的增殖,且诱导了其碱性成纤维细胞生长因子的表达.但是内皮素-1对自发性高血压大鼠血管平滑肌细胞增殖作用显著高于对Wistar-kyoto大鼠血管平滑肌细胞, 对它们碱性成纤维细胞生长因子表达作用也不相同,作用峰值分别为8倍和3 .5倍,且到达峰值时间不同,分别为8h和4h.且反义核酸对内皮素-1诱导的自发性高血压大鼠血管平滑肌细胞增殖的抑...