期刊论文

中文

中国医学工程

1672-2019

2008

16

1

26-28+31

10.3969/j.issn.1672-2019.2008.01.008

国家自然科学基金（No.30670634）；

本校为第一机构

目的在观察铀矿粉对体外培养的支气管上皮细胞的毒性作用同时,加入二甲亚砜,以了解其在支气管上皮细胞受铀矿粉染毒时的可能保护机制。方法将不同浓度的铀矿粉与体外培养的支气管上皮细胞进行长时间接触,分为对照组、染毒组和二甲亚砜（DMSO）保护组,通过血清抗性实验等,观察各组间细胞受损的情况。结果随着铀矿粉浓度的增高,支气管上皮细胞受损明显加重,加入二甲亚砜的支气管上皮细胞受损情况好于未加入者。DMSO对铀矿尘作用于BEAS-2B细胞后,可引起细胞DNA断裂,造成细胞拖尾率、彗星尾部DNA含量、尾长、尾部面积、尾距增加具有较好的抑制作用。结论铀矿粉对支气管上皮细胞有明显的恶性转化作用...

[Objective] The aim of this study was to explore the malignant transformation effect on cytotoxic of human bronchial epithelial cell （BEAS-2B） induced by uranium mine dust, further investigate the cause and mechanism of human lung cancer. All of these is to be researched the protection and mechanism of DMSO to cytotoxic, genotoxic, clastogenic and mutagenic which caused by uranium mine dust. [Methods] The immortalized human bronchial epithelial cells BEAS-2B were treated with uranium mine dust at the concentrations of d iffent dose, including...