期刊论文

Oxidative Stress Induced by Beryllium Sulfate on the Kidney of Mice

中文

实验动物科学

1006-6179

2012

29

6

13-16

10.3969/j.issn.1006-6179.2012.06.004

湖南省科技厅资助项目（No.2011TT2013） 湖南省研究生科研创新项目（No.CX20118382） 衡阳市科技局资助课题（No.2011ks11）

本校为第一机构

目的 探讨硫酸铍(BeSO4·4H2O)致小鼠肾脏氧化损伤的作用.方法 将36只6周龄雄性昆明种小鼠随机分成对照组、低剂量组及高剂量组,每组12只,对照组用灭菌生理盐水(0.9％氯化钠溶液)按0.1 mL/10 g(体质量)隔天腹腔注射,低、高剂量组分别以1 mg/kg BeSO4·4H2O和2 mg/kg BeSO4.4H2O生理盐水溶液隔天腹腔注射染毒,连续染毒4周.采集血液测定血清肌酐及尿素氮含量；计算肾脏脏器系数,采用差速离心法制备肾脏线粒体悬液,检测肾线粒体中活性氧(ROS)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性.结果 与对照组相比,硫酸铍暴露小鼠肾脏脏器系数显著降低,血清中肌酐及尿素氮含量...

Objective To probe into the effects of oxidative stress induced by beryllium sulfate （BeS04 ~ 4H20 ） for mice kidney. Method The thirty-six mice were randomly divided into three groups and administered with physiological saline of beryllium sulfate （ 1 mg/kg, 2 mg/kg） by peritoneal injection once every other day for a month. Then, kidney mitochondria were isolated by differential centrifugation, the organ coefficient of kidney was calculated. The levels of serum creatinine and serum BUN were detected. The Reactive Oxygen Species （ROS） and...