期刊论文

中文

中国动脉硬化杂志

1007-3949

2020

28

5

429-434

10.3969/j.issn.1007-3949.2020.05.012

湖南省科技计划项目（2015JC3081）；

本校为第一机构

目的 研究二甲双胍对肝细胞脂质蓄积的影响及作用机制.方法 体外培养人源Huh7肝细胞,细胞随机分为空白对照组及5 mmol/L二甲双胍、10 mmol/L二甲双胍、15 mmol/L二甲双胍处理组,确定最佳浓度后,使用LDL荷脂,然后分为空白对照组、LDL处理组和LDL+ 15 mmol/L二甲双胍处理组.实验终点,采用细胞增殖及毒性检测试剂盒检测细胞存活率,油红0染色和BODIPY脂质探针检测细胞内脂质含量,Dil-LDL检测细胞脂质摄取能力,Western blot分析固醇调节元件结合蛋白2(SREBP-2)、SREBP-1c、低密度脂蛋白受体(LDLR)和前蛋白转化酶枯草溶菌素9(PCSK9)蛋白的表达情况.结果 二甲双胍可抑制LDL诱导的肝细胞脂质蓄积,降低SR...

Aim To investigate the effect and mechanism of metformin on lipid accumulation in Huh7 hepatocytes.Methods Huh7 cells were cultured in vitro and divided into:blank control group,5 mmol/L,10 mmol/L and 15 mmol/L metformin treatment group.After determing the optimal concentration,LDL was used to load fat,and then divided into:blank control group,LDL group,LDL+15 mmol/L metformin treatment group.Cell proliferation and toxicity test kits were used to detect cell survival rate,oil red O and BODIPY lipid probes were used to detect intracellular lipid content,Dil-LDL was used to detect cell lipid upt...