期刊论文

Nuciferine promotes ABCA1 expression and cholesterol efflux in THP-1 macrophage-derived foam cells and its mechanism

中文

中国动脉硬化杂志

1007-3949

2018

26

9

872-876+924

10.3969/j.issn.1007-3949.2018.09.002

国家自然科学基金项目（81770461）；

本校为第一机构

目的观察荷叶碱（NF）对巨噬细胞源性泡沫细胞ATP结合盒转运体A1（ABCA1）表达和胆固醇流出的影响及机制。方法体外培养人源性THP-1单核细胞,使用佛波酯（160 nmol/L）对其进行诱导,促进其分化为巨噬细胞,并在50 mg/L氧化低密度脂蛋白处理下,使其荷脂形成泡沫细胞,进行常规体外培养细胞。高效液相色谱检测泡沫细胞内脂质成分,液体闪烁计数仪检测泡沫细胞胆固醇流出的水平;实时荧光定量PCR和Western blot检测ABCA1表达情况。PPARγ抑制剂GW9662和LXRα抑制剂GGPP分别处理泡沫细胞,通过荧光定量PCR和Western blot检测荷叶碱对泡沫细胞PPARγ、LXRα和ABCA1表达的影响。结果荷叶碱显著减少泡沫细胞...

Aim To investigate the effects and mechanisms of nuciferine（NF） on the expression of ATP binding cassette transporter A1（ABCA1） and cholesterol efflux in THP-1 macrophages-derived foam cells. Methods THP-1 cells were differentiated into macrophages by 160 nmol/L phorbol myristate acetate. THP-1 derived macrophages were incubated with 50 mg/L oxidized low density lipoprotein to induce foam cells and cultured with typical approaches. Lipids contents were tested with high performance liquid chromatogram. Liquid scintillation counter was used t...