期刊论文

Ezetimibe promotes hepatic uptake of low density lipoprotein cholesterol by up-regulating the expressions of sterol regulatory element binding protein-2 and low density lipoprotein receptor Ezetimibe promotes hepatic uptake of low density lipoprotein cholesterol by up-regu- lating the expressions of sterol regulatory element binding protein-2 and low density lipoprotein receptor

中文

中国动脉硬化杂志

1007-3949

2018

26

1

7-13

10.3969/j.issn.1007-3949.2018.01.002

81600291:国家自然科学基金 81773736:国家自然科学基金

本校为其他机构

目的 探讨依泽替米贝对肝脏低密度脂蛋白胆固醇（LDLC）摄取的影响及其机制。 方法 使用5 mg/（kg·d）依泽替米贝灌胃高脂模型小鼠,4个月后测定其血液中总胆固醇（TC）、LDLC和高密度脂蛋白胆固醇（HDLC）的含量；HE染色观察肝脏组织形态。依泽替米贝（30 μmol/L）与低密度脂蛋白（25 mg/L）共同孵育肝脏HepG2细胞24 h,油红O染色观察细胞的脂滴分布情况；酶学方法检测细胞内TC、游离胆固醇（FC）和胆固醇酯（CE）的含量；采用DiI-LDL检测细胞对LDLC的摄取情况；实时荧光定量PCR和Western blot分别检测甾醇调控元件结合蛋白2（SREBP-2）和低密度脂蛋白受体（LDLR）在mRNA及蛋白水平的表达；流式...

Aim To investigate the effect of ezetimibe on hepatic low density lipoprotein cholesterol （LDLC） uptake and its mechanism. Methods The hyperlipidemia model mice had intragastric administration of 5 mg/（kg·d） ezetimibe. After four months, the contents of total cholesterol （TC）, LDLC and high density lipoprotein cholesterol （HDLC） were measured in the blood of mice, and liver tissue morphology was observed by HE staining. HepG2 cells were incubated with ezetimibe （30 μmol/L） and low density lipoprotein （25 mg/L） for 24 hours. The di...