期刊论文

中文

中国动脉硬化杂志

1007-3949

2017

25

9

879-884

10.3969/j.issn.1007-3949.2017.09.003

国家自然科学基金项目（81570408、81370377和81470569） 南华大学研究生创新课题（2016XCX04）.

本校为其他机构

目的 观察亲环素A (CypA)对THP-1源性泡沫细胞三磷酸腺苷结合盒转运体A1 (ABCAl)表达和胆固醇流出的影响及机制.方法 160 nmol/L佛波酯诱导人THP-1单核细胞分化为巨噬细胞,与50 mg/L氧化型低密度脂蛋白(ox-LDL)共孵育,使其荷脂形成泡沫细胞,常规体外培养细胞;实验分为对照组和CypA处理组;液体闪烁计数仪检测细胞胆固醇流出水平,高效液相色谱检测细胞内脂质成分,实时荧光定量PCR和Western blot检测ABCA1表达,Western blot检测核因子κB (NF-κB)核转位水平;NF-κB抑制剂小白菊内酯抑制NF-κB活化;脂质体2000转染CD147 siRNA至THP-1源性泡沫细胞.结果 CypA显著抑制THP-1源性泡沫细胞胆固醇流出,促...

Aim To investigate the effects and mechanisms of cyclophilin A （CypA） on the expression of ATP binding cassette trasporter A1 （ABCA1） and cholesterol efflux in THP-1 derived macrophages. Methods THP-1 cells were incubated with 160 nmol/L phorbol ester to transform into macrophages. THP-1 derived macrophages were exposed to 50 mg/L oxidized low density lipoprotein （ox-LDL） and cultured with typical approaches. Liquid scintillation counter was used to determine the efficiency of cholesterol efflux. Lipids contents were tested with high perf...