期刊论文

The Effect of Interleukin-4 on ATP-binding Cassette Transporter A1 Expression and Cholesterol Efflux in THP-1 Macrophage

中文

中国动脉硬化杂志

1007-3949

2015

23

12

1203-1209

81170278:国家自然科学基金 81370377:国家自然科学基金 2015JJ2131:湖南省自然科学基金面上项目 B2014-070:湖南省卫生厅医药卫生科研项目

本校为第一机构

目的 探讨白细胞介素4(IL-4)对人类单核细胞株THP-1巨噬细胞源性泡沫细胞三磷酸腺苷结合盒转运体A1(ABCA1)表达及细胞内胆固醇流出的影响及机制.方法 THP-1巨噬细胞源性泡沫细胞加入不同浓度的IL-4,处理不同时间,以及加入肝X受体α(LXRα)激动剂T0901317处理后,采用半定量RT-PCR和Western blot分别检测细胞ABCA1及LXRα mRNA和蛋白质的表达,采用油红O染色观察细胞内脂质蓄积情况,使用液体闪烁计法检测细胞内胆固醇流出,采用高效液相色谱法检测细胞内总胆固醇、游离胆固醇和胆固醇酯的量.结果 IL-4能呈浓度和时间依赖性地降低THP-1巨噬细胞源性泡沫细胞ABCA1表达(P＜0.05),并减少细胞内胆固醇流出...

Aim To investigate the effects of IL-4 on ATP-binding cassette transporter A1( ABCA1) expression and cholesterol efflux in human THP-1 macrophage-derived foam cells. Methods THP-1 macrophage-derived foam cells were treated with different concentrations of IL-4,different time,and liver X receptor α( LXRα) agonist T0901317.Semi quantitative RT-PCR and Western blot were used to detect the expressions of ABCA1,LXRα mRNA and protein respectively. Lipid accumulation in cells was observed by oil red O staining. Intracellular cholesterol efflux was ...