期刊论文

中文

中国动脉硬化杂志

1007-3949

2014

22

5

448-452

湖南省自然科学基金资助项目（14JJ2091） 湖南省教育厅科研资助项目（12C0339）

本校为第一机构

目的 探讨miR-19b对三磷酸腺苷结合盒转运体A1(ABCA1)介导巨噬细胞胆固醇流出的影响及其机制.方法 将miR-19b mimic或miR-19b inhibitor转染入THP-1源性巨噬细胞,液体闪烁计数仪检测细胞内胆固醇流出,油红O染色观察细胞内脂滴情况,生物信息学分析miR-19b与ABCA1 3'UTR的结合关系,荧光素酶报告基因检测miR-19b与ABCA1的结合情况,Westem blot检测ABCA1蛋白水平.结果 miR-19b抑制巨噬细胞胆固醇流出,增加胞内脂质蓄积和泡沫细胞形成,而anti-miR-19b则刚好出现相反的结果.miR-19b与ABCA1 3'UTR的3110-3116位结合,且二者结合的自由能较低.miR-19b显著抑制荧光素酶活性及其巨噬细胞中ABCA1蛋白的表达....

Aim To investigate the effect and mechanism of miR-19b on ATP binding cassette transporter A1 （ABCA1） mediated intracellular cholesterol efflux. Methods After transfected miR-19b mimic and inhibitor into THP-1 derived macrophage, cholesterol efflux was detected with liquid scintillator, and intracellular lipid droplet was stained with oil red O. The binding of miR-19b with ABCA1 3＇UTR was analyzed with bioinformatics websites. MiR- 19b binding to ABCA1 3＇UTR was confirmed with luciferase reporter assay. ABCA1 expression was measured by West...