期刊论文

中文

中国动脉硬化杂志

1007-3949

2014

22

7

680-684

湖南省自然科学基金资助项目（14JJ2091） 湖南省教育厅资助科研项目（12C0339）

本校为第一机构

目的探讨薯蓣皂苷元（Dgn）对巨噬细胞ATP结合盒转运体A1（ABCA1）表达及其介导胆固醇流出的影响。方法用Dgn或结合ABCA1小干扰RNA（siRNA）处理THP-1源性巨噬细胞24 h后,Western blot检测ABCA1蛋白水平,HPLC检测胞内总胆固醇（TC）、游离胆固醇（FC）及胆固醇酯（CE）含量,液体闪烁计数仪检测胞内胆固醇流出,油红O染色观察胞内脂滴情况。结果 Dgn呈浓度依赖性上调巨噬细胞ABCA1的表达,促进胞内胆固醇流出,降低胞内TC、FC和CE含量,抑制胞内脂质蓄积和泡沫细胞形成。而ABCA1 siRNA与Dgn共处理细胞后,明显抑制Dgn促胆固醇流出作用,胞内脂质蓄积和泡沫细胞形成明显加剧。结论 Dgn通过促进巨噬细胞A...

Aim To investigate the effect of diosgenin（Dgn） on ABCA1 expression and cholesterol efflux in THP-1 derived macrophage. Methods ABCA1 expression was detected by Western blot. Cholesterol efflux was detected with liquid scintillator; intracellular total cholesterol（TC）,free cholesterol（FC） and cholesterol ester（CE） were determined with HPLC; and intracellular lipid droplet was stained with oil red O in THP-1-derived macrophage treated with Dgn,alone or in combination with ABCA1 siRNA. Results Dgn upregulated macrophage ABCA1 expression i...