期刊论文

中文

中国动脉硬化杂志

1007-3949

2009

17

5

354-358

2006CB504509:国家重点基础研究发展计划（973计划）

本校为其他机构

目的 探讨应激性血压升高对下丘脑肾上腺髓质素编码基因表达及水平的影响.方法 58只雄性SD大鼠随机分为对照组(n=23)和应激组(n=35),应激组给予噪声或噪声加足底电击随机组合输出刺激制备应激性高血压动物模型.48只动物在实验第1、5、10、15天以及实验结束后5、10天测量尾动脉压后处死(每个时间点对照组和应激组分别为n=3和n=5).分离下丘脑并制备组织匀浆后分别采用逆转录聚合酶链反应检测肾上腺髓质素编码基因表达变化,放射免疫法检测肾上腺髓质素水平变化.另外10只在实验第10天后断头取下丘脑制备冰冻切片,采用免疫组织化学法检测肾上腺髓质素免疫阳性细胞分布变化.结果 应激5天后动物血压开始...

Aim To investigate the dynamic changes of adrenomedullin mRNA expression and its level in the hypothalamus of stress-induced-hypertensive rats. Methods 58 male SD rats were divided into control group （ n = 23 ） and stress group （n = 35） receiving noise or noise with electric foot shock randomly combined and output by a computer for 15 days. 48 rats were executed and their hypothalamus were separated to monogenate individually at stress 1, 5, 10 and 15 days and post-stress 5 and 10 days （for each subgroup n = 3 or n = 5 ）. The changes of t...