期刊论文

中文

中国动脉硬化杂志

1007-3949

2007

15

7

491-493

10.3969/j.issn.1007-3949.2007.07.004

中国博士后基金（2005038472）资助；

本校为其他机构

目的 观察基质细胞衍生因子1α对体外培养的大鼠骨髓源内皮祖细胞迁移的影响.方法 微孔法从大鼠骨髓提取内皮祖细胞.免疫荧光鉴定血管内皮生长因子受体2和CD133, 不同浓度基质细胞衍生因子1α处理内皮祖细胞后,采用Transwell迁移系统检测内皮祖细胞迁移能力.结果 分离出的大鼠骨髓内皮祖细胞免疫荧光下血管内皮生长因子受体2+和CD133+双阳性, 基质细胞衍生因子1α呈浓度依赖性促进内皮祖细胞迁移,对照组与基质细胞衍生因子1α各处理组比较差异均具显著性(P＜0.05或P＜0.01).结论 基质细胞衍生因子1α呈浓度依赖性促大鼠骨髓源内皮祖细胞迁移.

　Aim To study whether stromal cell-derived factor-1α （SDF-1α） have effects on endothelial progenitor cells （EPC） and its preliminary mechanism. Methods Bone marrow derived EPC were aquried by Micropore-method and characterized by immunofluorescence staining. The abilities of migration were detected by the methods of transwell migration after EPC treated with different concentrations of SDF-1α. Results SDF-1α promoted EPC biologic activity of migration in a concentration-dependent manner （P〈0.05 or P〈0.01）...