期刊论文

中文

中国动脉硬化杂志

1007-3949

2007

15

7

494-496

10.3969/j.issn.1007-3949.2007.07.005

中国博士后基金（2005038472） 教育部重点科技基金（104158） 湖南省自然科学基金（06jj5051）联合资助

本校为第一机构

目的 探讨pcDNA3.1-基质细胞衍生因子1α重组质粒对单核细胞的趋化作用.方法 将pcDNA3.1-基质细胞衍生因子1α质粒用脂质体的方法导入人脐静脉内皮细胞株ECV304细胞中,G418 筛选细胞克隆.通过逆转录聚合酶链反应法在转录水平检测基质细胞衍生因子1α在转基因ECV304细胞中的表达.通过Transwell实验研究基质细胞衍生因子1α对单核细胞的趋化作用.结果 获得了稳定表达基质细胞衍生因子1α基因的ECV304细胞克隆,pcDNA3.1-基质细胞衍生因子1α重组质粒对单核细胞具有趋化作用.结论 重组大鼠基质细胞衍生因子1α对单核细胞具有明显的趋化作用.

Aim To investigate the effect of pcDNA3.1-SDF-1α plasmid on THP-1 cells chemotaxis. Methods pcDNA3.1-SDF-1α plasmid was transfected into ECV304 cells by lipofectamine and the positive clones were screened by G418. The expression of SDF-1α gene in the transfected ECV304 cells were detected by RT- PCR. Then explored the effect of SDF-1α on THP-1 cells chemotaxis with transwell permeable supports. Results G418-resistant ECV304 cell clones were obtained. pcDNA3.1-SDF-1α plasmid effect on THP-1 cells migration. Conclusion SDF-1α re...