期刊论文

中文

中国动脉硬化杂志

1007-3949

2006

14

9

759-762

10.3969/j.issn.1007-3949.2006.09.006

第38批博士后基金（#2005038472）；

本校为第一机构

目的 探讨基质细胞衍生因子1α对大鼠血管平滑肌细胞与单核细胞粘附的影响.方法 原代培养的大鼠血管平滑肌细胞与50 mg/L氧化型低密度脂蛋白孵育48 h,将单核细胞与平滑肌细胞共孵育后洗脱检测粘附功能,并用基质细胞衍生因子1α抗体阻断.结果 经氧化型低密度脂蛋白处理的血管平滑肌细胞基质细胞衍生因子1α上调5倍,单核细胞粘附增加29倍,但可被基质细胞衍生因子1α单抗所阻断.结论 基质细胞衍生因子1α参与单核细胞与大鼠血管平滑肌细胞的粘附过程.

Ahn To explore the effect of stromal cell-derived factor-1α （SDF-1α） on rat vascular smooth muscle cells （VSMC） /monocytes adhesion. Methods VSMC was ineuhated with different concentrations of oxidized low density lipoprotein （ox-LDL）, SDF-1α mRNA and protein expression were revealed by reverse transcription polymerase chain reaction （ RT-PCR） and Western blotting. VSMC pretmated with 50 mg/L ox-LDL for 48 h were incubated with monocytes and SDF-1α antibody to observe cells adhesion. Results Compared with control, SDF-1α was upregu...