期刊论文

中文

中国动脉硬化杂志

1007-3949

2006

14

2

170-174

10.3969/j.issn.1007-3949.2006.02.022

纽约中华医学基金会（CMB）资助（编号82-412）；

本校为其他机构

的探讨肥胖者高密度脂蛋白亚类组成与脂蛋白酯酶基因内含子8 HindⅢ酶切位点多态性的关系.方法采用聚合酶链反应限制性片长多态性和双向电泳免疫印迹检测法,分析95例肥胖者和144例体质指数正常者的脂蛋白酯酶基因内含子8 HindⅢ多态性、高密度脂蛋白亚类组成及相对百分含量.结果肥胖组和对照组均以H+H+基因型为主.肥胖组血浆甘油三酯、载脂蛋白B100、CⅡ、CⅢ、E、前β1高密度脂蛋白水平及甘油三酯/高密度脂蛋白胆固醇较对照组增加,而高密度脂蛋白胆固醇、载脂蛋白AI、高密度脂蛋白2b水平及载脂蛋白E/CⅢ明显降低(P

Aim To elucidate the relationship between hpoprotein lipase （LPL） gene HindⅢ polymorphism and plasma lipid profides and HDL subclasses in obesity. Methods LPL gene Hind Ⅲ polymorphism was assayed by polymerase chain reaction-restriction fragnent length polymorphism （ PCR-RFLP）. The subclasses of serum HDL in 95 obese subjects and 144 nonobese subjects were determined by two-dimensional gel electrophoresis conjunction with immtmodetection method. Results Both in obese group and control group, the H^＋ H^＋ homozygote and H^＋ allele were t...