期刊论文

中文

中国动脉硬化杂志

1007-3949

2005

13

2

143-145

10.3969/j.issn.1007-3949.2005.02.005

国家重点基础研究发展计划(973计划)

本校为其他机构

目的观察血小板活化因子受体拮抗剂WEB2086对载脂蛋白E基因敲除鼠斑块内血管新生和斑块面积的影响.方法8周龄雄性载脂蛋白E基因敲除鼠普通饲料喂养24周,然后随机分为对照组和WEB2086组2组,每组18只.两组均普通饲料,对照组普通饮水,WEB2086组则在普通饮水中加入血小板活化因子受体拮抗剂WEB2086(43 mg/L).继续喂养8周后,空腹18 h采血,安乐死,取材.全自动生物化学分析仪检测血清脂质含量,CD31全层铺片检测斑块内毛细血管密度,含斑块的主动脉环置于matrigel中培养检测斑块新生毛细血管的能力,苏丹Ⅳ染色检测斑块面积.结果WEB2086对血清脂质无明显影响.WEB2086明显抑制粥样斑块内毛细血管平均密度(3...

Aim To investigate the effect of platelet activating factor receptor (PAFR) antagonist on angiogenesis in plaque and lesion area of apolipoprotein E-deficient mice (apo E -/-). Methods 8-week-old male apo E -/- mice were fed a standard chow diet for 24 weeks to develop advanced lesions. Mice were then separated into the following two groups (n=18) and treated for 8 weeks: (1) chow diet with regular water, (2) chow diet with WEB2086 in the water (4.3 mg in 100 mL). Whole-Mount CD31 Stain and Plaque-Associated Sprouting have been used to estimate...