期刊论文

中文

中国动脉硬化杂志

1007-3949

2005

13

3

292-296

10.3969/j.issn.1007-3949.2005.03.011

湖南省重点科技项目（01SSY1003）资助；

本校为其他机构

目的 目前,肥大细胞与动脉粥样硬化的关系研究主要是病理学观察和细胞学实验.基于此种现状,本课题拟用化合物48/80探讨肥大细胞脱颗粒与载脂蛋白E基因敲除小鼠主动脉斑块的关系.方法 40只10周龄载脂蛋白E基因敲除小鼠给予高脂高胆固醇饲养至16周龄,随机分为2组(n=20),实验组给予腹腔注射化合物48/80(0.5 mg/kg),隔日一次,共4次;对照组腹腔注射D-hank's.第4次注射后半小时,安乐死处死动物,摘眼球取血,分离血清,采用酶法测定血清脂质含量,采用比色法测定血清类胰蛋白酶活性浓度.原位灌流固定,取主动脉置10%中性缓冲***中固定,石蜡包埋,连续切片,HE染色、甲苯胺蓝染色以及α-平滑肌肌动蛋白、...

Aim So far, research on the relationship of mast cells, atheroselerosis and angiogencsis is mostly pathological observation and cytology experiments. Based on the status, compound 48/80 and apolipoprotein E-knockout mice were used to investigate the effect of mast cell degranulation on plaque. Methods 40 apoE-/- mice were fed a Western-type diet from 10wecks old. At 16wecksold, mice were divided into 2 groups and treated for 8 days as follows ： group I, mice were intraperitoncal injected with compound 48/80, 0.5 mg/kg, every other day; and gro...