期刊论文

中文

中国动脉硬化杂志

1007-3949

2005

13

3

301-304

10.3969/j.issn.1007-3949.2005.03.013

01JJY2145:湖南省自然科学基金 4-02-JY-02C387:湖南省教育厅科学研究项目

本校为第一机构

目的 研究细胞质膜小凹/小凹蛋白1在动脉粥样硬化病变中的表达变化,探索动脉粥样硬化发生过程中细胞胆固醇逆转运障碍的机制.方法 采用高脂饲料喂养动脉粥样硬化敏感C57BL/6J小鼠24周,计算机图像分析系统计算动脉粥样硬化病灶面积和主动脉内膜和中膜厚度;电镜观察小凹在主动脉病变区的血管平滑肌细胞表达,Western印迹检测血管壁小凹蛋白1变化情况.结果 动脉粥样硬化模型小鼠主动脉病变区的血管平滑肌细胞膜上存在有小凹结构,但排列稀疏,与正常血管平滑肌细胞相比明显减少,泡沫样改变的血管平滑肌细胞未见明显小凹结构的存在.通过Western印迹检测观察到,高脂组血管壁的小凹蛋白1蛋白表达量明显减...

Aim To investigate whether caveolae/caveolin-1 participates in the progression of atheresclerosis. Methods C57BL/6J mice were fed with normal chow plus 1.5% cholesterol and 15% lord for 24 weeks. The area of atherosclerotic plaque, thickness of intima and media of aorta were determined by graphic analysis computer system; Electron microscopy was used to investigate the expression of caveolae in vascular smooth muscle cell （VSMC） ; the expression of caveolin-1 in vascular wall was detected by western blot. Results The serum levels of total cho...