期刊论文

中文

中国动脉硬化杂志

1007-3949

2004

12

5

524-528

10.3969/j.issn.1007-3949.2004.05.007

湖南省卫生厅科研项目

本校为第一机构

为探讨高密度脂蛋白2和高密度脂蛋白3对THP-1巨噬细胞过氧化体增殖物激活型受体γ和CD36表达及脂质蓄积的影响.取新鲜抗凝血浆,用超速离心术进行密度梯度离心,收集低密度脂蛋白、高密度脂蛋白2和3.将氧化型低密度脂蛋白分别与高密度脂蛋白2和3共孵育THP-1巨噬细胞,用油红O染色及高效液相分析法观测细胞内脂质蓄积程度;用逆转录聚合酶链反应检测CD36和过氧化体增殖物激活型受体γ mRNA的表达;用Western blotting检测CD36和过氧化体增殖物激活型受体γ蛋白的表达.结果发现,与单独用氧化型低密度脂蛋白和THP-1孵育相比,用高密度脂蛋白2、高密度脂蛋白3分别与氧化型低密度脂蛋白共孵育THP-1可使细胞...

Aim To explore the effects of high density lipoprotein 2 (HDL 2) and 3 (HDL 3) on expression of peroxisome proliferator-activated receptor γ (PPARγ)and CD36, and cellular lipid accumulation in THP-1 macrophages. Methods After exposure of the cultured THP-1 macrophages to oxidized low density lipoprotein (ox-LDL) only, and ox-LDL and HDL 2 or HDL 3, lipid accumulation was valued by high performance liquid chromatography combined with enzyme assay. The mRNA and protein expression levels of PPARγ and CD36 were determined by reverse transcriptio...