期刊论文

中文

中国动脉硬化杂志

1007-3949

2004

12

1

27-30

10.3969/j.issn.1007-3949.2004.01.007

本校为第一机构

为研究苦瓜蛋白对泡沫细胞形成的作用及其机制,用离心、超滤和色谱技术从苦瓜(Momordica charantia L)果肉中提取苦瓜蛋白(Momordicin),THP-1细胞经160 nmol/L PMA孵育24 h,诱导其分化成巨噬细胞,然后用25 mg/L乙酰化低密度脂蛋白孵育48 h,使其泡沫化,以观察苦瓜蛋白对泡沫细胞形成的影响.用高效液相色谱法分析细胞内胆固醇和胆固醇酯的变化,用逆转录-聚合酶链反应技术检测苦瓜蛋白对三磷酸腺苷结合盒转运体A1的影响.结果发现,经苦瓜蛋白作用后,细胞内总胆固醇及胆固醇酯均显著减少(对照组总胆固醇为634±21,苦瓜蛋白处理组为257±26, P

Aim To study the inhibitory effect and mechanisms of Momordicin on foam cell. Methods Momordicin (28 kDa) was purified from the juice of Momordica charantial by eccentricity, super-filtration and chromatography technology. After incubated with 160 nmol/L PMA for 24 h, THP-1 was induced to macrophage, this macrophage was induced to foam cell by incubated with 25 mg/L ac-LDL for 48 h, and the effect of Momordicin on foam cell was measured, cholesteryl and cholesteryl ester within THP-1 were measured by high performance liquid chromatography (HPLC), and regulated effect of Momordicin on ATP bindi...