期刊论文

中文

中国动脉硬化杂志

1007-3949

2003

11

2

107-110

10.3969/j.issn.1007-3949.2003.02.004

国家自然科学基金 (30 0 70 934)资助；

本校为其他机构

为研究苦瓜蛋白对凋亡的关键酶半胱天冬酶3及凋亡的调节作用,用已经建立起来的方法从苦瓜果肉中提取苦瓜蛋白.将BALB/C小鼠分为4组:病毒对照组、正常对照组、药物对照组和药物治疗组,分别在第0天、第3天、第7天和第14天各处死5只小鼠,第21天全部处死动物,心肌组织半胱天冬酶3活性测定按照CALBIOCHEM公司的试剂说明书进行,并稍加改正,凋亡鉴定按照Oncogene公司的TdT-DNA裂解片断末端原位标记试剂说明书进行.结果发现,①病毒对照组第7天开始出现半胱天冬酶3活性(0.63±0.21 pmol/min,n=5),第14天的活性(10.9±1.5 pmol/min,n=5)显著高于第7天,第21天的活性(12.6±1.3 pmol/min,n=5)又高于第14天.②...

Aim To measurement the inhibitory effect of Momordicin on the activity of caspase 3 and apoptosis of myocardium in BALB/C mice with coxsackievirus B3 myocarditis. Method Momordicin was purificated from Momordica charantia L by our previous reported methods. Four group animals were used in this experiment, such as disease group (DG), normal group (NG), Momordicin group (MG), and Momordicin therapy group (MTG). Five animals were killed at the 3rd,the 7th,and the 14th days in each group, and all were killed at the 21st day. According to the method...