期刊论文

中文

中国动脉硬化杂志

1007-3949

2003

11

4

325-329

10.3969/j.issn.1007-3949.2003.04.012

本校为其他机构

为探讨过氧化体增殖物激活型受体γ和δ在高密度脂蛋白介导的细胞胆固醇流出中的作用.取新鲜抗凝血浆,用超速离心机进行密度梯度离心,收集密度为1.063～1.210组分.U937细胞用50～400 nmol/L反义过氧化体增殖物激活型受体γ和δ预处理细胞12 h.培养U937细胞与0.28 mCi/L [3 H]-胆固醇乙醇液共孵育24 h,1 500 r/min离心10 min,收集细胞,PBS漂洗2次,RPMI-1640重悬细胞,加HDL继续培养0～48 h.液体闪烁计数仪测细胞相对放射活性.Western blot检测过氧化体增殖物激活型受体γ和δ蛋白表达水平.不同浓度HDL处理后细胞胆固醇流出具有明显差异,呈剂量-效应关系.用100 nmol/L反义过氧化体增殖物激活型受体...

Aim This study examines the relationship between PPAR gamma and delta and cholesterol efflux mediated by high density lipoprotein. Methods Pooled plasma were collected from health human. Component (density 1.063 1.21 g/cm 3) was prepared by density ultracentrifugation. U937 cells were treated by cholesterol for 24 h, cell pellets were washed with PBS, then added RPMI 1640 supplemented with 1% FBS. The cells were treated by the antisense PPAR gamma and delta oligo DNA, PPAR gamma activator ciglitazone, and PPAR responsive element decoy, respectively. The effects of the oligo DNA on efflux of ch...