期刊论文

中文

中国动脉硬化杂志

1007-3949

2003

11

4

304-308

10.3969/j.issn.1007-3949.2003.04.007

湖南省科技厅科研项目

本校为其他机构

以THP-1细胞源泡沫细胞为研究对象,观察三磷酸腺苷结合盒转运体A1在单核-巨噬细胞源泡沫细胞胆固醇流出中的作用.实验采用流式细胞术检测的方法,来判断三磷酸腺苷结合盒转运体A1激动剂22(R)-羟基胆固醇和抑制剂4,4-二异硫氰酸二丙乙烯2,2-二磺酸(4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, DIDS)对THP-1细胞源泡沫细胞三磷酸腺苷结合盒转运体A1表达的影响.实验结果发现,22(R)-羟基胆固醇作用THP-1细胞源泡沫细胞不同时间后,能明显增加细胞三磷酸腺苷结合盒转运体A1的蛋白表达, 流式细胞术检测三磷酸腺苷结合盒转运体A1表达的平均荧光强度从0 h的31.1增加到24 h的45.2;而DIDS作用THP-1细...

Aim To study the action of ATP binding cassette transporter A1 (ABCA1) on cholesterol efflux in THP 1 macrophage derived foam cell. Methods After exposure of the cultured THP 1 macrophage derived foam cell to 22(R) hydroxycholesterol and 4,4' diisothiocyanostilbene 2,2' disulfonic acid (DIDS) at different time, cholesterol efflux and ABCA1 protein level were determined by FJ 2107P type liquid scintillator and flow cytometer, respectively. Results 22(R) hydroxycholesterol increased cholesterol efflux in THP 1 macrophage derived foam cell with time dependent pattern and DIDS inhibited cholestero...