期刊论文

中文

中国动脉硬化杂志

1007-3949

2001

9

5

385-387

10.3969/j.issn.1007-3949.2001.05.005

湖南省自然科学基金 （ 0 0JJY2 0 2 7）； 湖南省教育厅课题 （ 0 0C15 1）资助；

本校为第一机构

研究氧化型低密度脂蛋白诱导人单核细胞U937细胞吞噬脂质发生凋亡过程中对细胞间粘附分子-1表达的影响.用胸腺嘧啶核苷将U937细胞阻滞在G1期,流式细胞仪监测同步化处理效果;流式细胞术检测U937细胞泡沫化过程中细胞凋亡和细胞间粘附分子-1的表达;逆转录-聚合酶链反应分析细胞间粘附分子-1 mRNA的表达.结果显示,80 mg/L氧化型低密度脂蛋白温育U937细胞48 h可形成典型的泡沫细胞,72 h可见凋亡细胞增多;氧化型低密度脂蛋白温育U937细胞12 h即可检测到细胞间粘附分子-1表达,24 h时达到最高值,48 h 略有降低,72 h时则明显降低.结果表明,氧化型低密度脂蛋白可以促进U937细胞细胞间粘附分子-1表达,细胞...

Aim To explore the time course of expression of ICAM-1 gene and protein in macrophages foamy process induced by ox-LDL. Methods Cell cycle of Human myeloid leukemia U937 cells was arrested in G1 by preincubated with thymidine (2.0 mmol/L) for 24 h, then cells released at the same time. U937 cells were incubated with ox-LDL (80 mg/L) for time course(12 h, 24 h, 48 h or 72 h) treatment. Results Flow cytometric analysis results revealed that ICAM-1 protein expression increased from 12 h to 24 h and was highest at 24 h, but ICAM-1 decreased at 48 h. RT-PCR results showed that ICAM-1 mRNA level inc...