期刊论文

Detection of Trace Taurine, the Composition of Antiatherosclerosis, in Animal Tissues

中文

中国动脉硬化杂志

1007-3949

1997

04

318-322

本校为第一机构

为测定动物组织中对预防动脉粥样硬化有效的成分-牛磺酸的含量．利用渗透压原理，以水为溶剂先提取动物组织中牛磺酸，用真空冷冻抽干浓缩后进行柱前衍生处理、进样．经反相高效液相色谱柱内疏水分离后，再用标准曲线法进行定量测定。在选定的试验条件下，牛磺酸保留时间为5．06min，分离效果好，峰形呈正态分布，样品溶液相对标准偏差为1．5％．最低检出量是4.48ng，牛磺酸含量在5．58X103～1．79X105μg／L范围，色谱峰面积与牛磺酸含量间有良好线性关系（r=0．9998），131．2～9682ng牛磺酸的样品加标回收率为97．79／～101．99％。按照此法测得蛞蝓组织中牛磺酸含量为75．4μg／g。此方法简...

Aim To develop a new method for microdetermination of taurine the composition of antiatherosclerosis, in animal tissues With reverse-phase high performance liquid chromatography (RP-HPLC) Methods Animal tissues were soaked in water to extract taurine. The extraction was concentrated by vacuum freezing and drying. Then using the mixed liquid of Phenylisothiocyanate (PITC), triethylamine,water and ethanol (1: 1:1: 7) as derivative to precolumn derived taurine with PITC. Under the following conditions: 1 mL/min flow rate, 254 nm wavelength and col...