期刊论文

Panax notoginseng saponin relieves LPS-induced depression-like behavior by inhibiting activation of astrocytes in mice

中文

解剖学杂志

1001-1633

2019

42

1

26-30

10.3969/j.issn.1001-1633.2019.01.007

2018JJ4034:湖南省自然科学基金省市联合项目 16A187:湖南省教育厅重点项目 16B245:湖南省教育厅优秀青年项目 17B230:湖南省教育厅优秀青年项目 2017XJXZ035:创新项目 2016NH056XJZ:创新项目

本校为第一机构

目的 :观察三七总皂苷（PNS）对脂多糖（LPS）诱导的小鼠抑郁样行为以及海马星形胶质细胞（AST）活化的影响。方法 :将小鼠随机分为生理盐水（NS）组、LPS组、PNS+LPS组。利用喷糖实验、糖水偏好实验及强迫游泳实验检测小鼠抑郁样行为,免疫组织化学显色观察胶质原纤维酸性蛋白（GFAP）的表达变化,免疫印迹检测海马GFAP、乙醛脱氢酶1蛋白家族L1（ALDH1L1）的表达变化,荧光定量PCR检测海马GFAP和肿瘤坏死因子-α（TNF-α）mRNA的表达。结果 :与NS组相比,LPS组舔糖潜伏期延长、糖水偏好百分比下降、强迫游泳不动时间增加,PNS预处理可逆转上述抑郁样行为;与NS组相比,LPS组海马GFAP阳性细胞数目增加,...

Objective:To explore the effect of panax notoginseng saponins(PNS)on lipopolysaccharide(LPS)-induced depression-like behavior and activation of astrocytes(AST)in mice.Methods:Male Kunming mice were randomly assigned to NS group,LPS group,and PNS+LPS group.Splash test,Sucrose preference test(SCPT)and force swimming test(FST)was used to observe the depression-like behavior.Expression of astrocyte marker GFAP and ALDH1L1 in the hippocampus was determined by immunohistochemistry and Western blotting.RT-qPCR was used to investigate the mRNA expressi...