AbstractConcerns over the health risk of the widely distributed, commonly used titanium dioxide nanoparticles (nano‐TiO2) are increasing worldwide. Yet, up‐to‐now, our understanding in their potential effects on the cardiovascular system is very limited and the toxicological mechanisms are still unclear. In the present study, the CCK‐8 assay was performed to determine the cytotoxicity of four sizes (10, 30, 50, and 100 nm) of anatase nano‐TiO2 on human umbilical vein endothelial cells (HUVECs) in culture, and the flow cytometry was employ...