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FGF21 induces autophagy-mediated cholesterol efflux to inhibit atherogenesis via RACK1 up-regulation

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成果类型:
期刊论文
作者:
Lin Xiaolong;Guo Dongmin;Liu, Mihua;Wang Zuo;Hu Huijun;...
通讯作者:
Zeng Zhaolin
作者机构:
[Lin Jun; Lin Wensheng; Hu Huijun; Hu XueMei; Tan Qiufen; Lin Xiaolong; Pan Yuping] Guangzhou Med Univ, Huizhou Third Peoples Hosp, Dept Pathol, Huizhou City, Peoples R China.
[Wang Zuo; Zeng Zhaolin; Guo Dongmin] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang City, Peoples R China.
[Liu, Mihua] Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Ctr Lipid Res, Chongqing, Peoples R China.
[Liu, Mihua] Chongqing Med Univ, Affiliated Hosp 2, Key Lab Mol Biol Infect Dis, Minist Educ,Inst Viral Hepatitis, Chongqing, Peoples R China.
[Liu, Mihua] Gannan Med Univ, Dept Lab Med, Affiliated Hosp 1, Ganzhou City, Peoples R China.
通讯机构:
[Zeng Zhaolin] C
Chongqing Med Univ, Nanchuan Peoples Hosp, Dept Cardiol, Chongqing 408400, Peoples R China.
语种:
英文
关键词:
cholesterol oleate;complementary DNA;fat droplet;fibroblast growth factor 21;high density lipoprotein cholesterol;lentivirus vector;low density lipoprotein cholesterol;receptor for activated C kinase 1;short hairpin RNA;small interfering RNA;triacylglycerol;AMPK signaling;animal cell;animal experiment;animal model;animal tissue;Article;atherosclerosis;autophagy (cellular);blood sampling;cell stress;cholesterol transport;controlled study;genetic transfection;high performance liquid chromatography;lipid blood level;lipid degradation;lipid diet;male;mouse;nonhuman;phase contrast microscopy;plasmid;priority journal;real time polymerase chain reaction;signal transduction;transmission electron microscopy;upregulation;Western blotting
期刊:
Journal of Cellular and Molecular Medicine
ISSN:
1582-1838
年:
2020
卷:
24
期:
9
页码:
4992-5006
基金类别:
This work was supported by grants from The National Natural Science Foundation of China (No. 81600342), The Medical Foundation of Hui Zhou (2019Y134), The Medical Research Foundation of Guangdong Province (A2018099), The Natural Science Foundation of Hunan Province (No.2018JJ3459) and The Education Department Foundation of Hunan Province (No.2016C1391).
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
Fibroblast growth factor 21 (FGF21) acts as an anti-atherosclerotic agent. However, the specific mechanisms governing this regulatory activity are unclear. Autophagy is a highly conserved cell stress response which regulates atherosclerosis (AS) by reducing lipid droplet degradation in foam cells. We sought to assess whether FGF21 could inhibit AS by regulating cholesterol metabolism in foam cells via autophagy and to elucidate the underlying molecular mechanisms. In this study, ApoE−/− mice were fed a high-fat diet (HFD) with or without FGF2...

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