期刊论文

中文

临床麻醉学杂志

1004-5805

2017

33

10

1021-1024

10.3969/j.issn.1004-5805.2017.10.021

国家自然科学基金（81301144） 国家留学基金（201608430251）

本校为第一机构

目的研究新生期七氟醚暴露对下丘脑-垂体-肾上腺皮质(hypothalamic-pituitary-adrenal, HPA)轴发育及功能的影响。方法新生雄性SD大鼠54只,6日龄,随机分为三组:对照组(C组)、七氟醚组(S组)和布美他尼+七氟醚组(BS组),每组18只。C组:吸入100%氧气6 h; S组:吸入2.1%七氟醚6 h;BS组:吸入2.1%七氟醚6 h,在麻醉前30 min和麻醉后3 h注射布美他尼。6 h后,每组随机选取6只处死收集血清,采用ELISA法测定皮质酮浓度;各组剩余大鼠于第60天行高架十字迷宫测试,第65天行束缚应激30 min,并在应激前后取尾静脉血,采用ELISA法测定血清皮质酮浓度。结果麻醉后S组血清皮质酮浓度明显高于C组(P

Objective To investigate the effects of sevoflurane exposure on the hypothalamicpituitary- adrenal(HPA)axis development in neonatal rats.Methods Fifty-four SD rats(P6)were randomly divided into three groups:control group(group C),sevoflurane group(group S)and bumetanide+ sevoflurane group(group BS),n=18 in each group.In group C,rats were treated with pure oxygen for 6 hours and rats in group S received 2.1% sevoflurane anesthesia for 6 h.Rats in group BS also received 2.1%sevoflurane anesthesia for 6 h,but they were injected with bumetanide before 30 min and at 3 hduring the anesthesia.6 rats ...