期刊论文

Effects of desflurane post-processing on the expression of glucose transporter 4in myocardial ischemiareperfusion injury in rabbits

中文

临床麻醉学杂志

1004-5805

2017

33

11

1099-1102

10.3969/j.issn.1004-5805.2017.11.016

湖南省卫生计生委课题（C2016084）；

本校为第一机构

目的研究地氟醚后处理对心肌缺血-再灌注损伤葡萄糖转运蛋白4(glucose transporter 4,GIUT_4)表达的影响。方法选择雄性新西兰白兔24只,体重4.0~5.0kg,随机分为四组:正常对照组(NC组)、缺血-再灌注组(IR组)、缺血-再灌注后处理组(IRP组)和地氟醚后处理组(Des组),每组6只。结扎左冠脉左室支建立心肌缺血-再灌注模型。分别于缺血前即刻(T_0)、缺血后30min(T_1)、再灌注后30min(T_2)、60min(T_3)和120min(T_4)时采集取耳缘静脉血检测血糖、胰岛素浓度和心肌葡萄糖摄取率;采用定量RT-PCR法检测心肌组织GLUT_4mRNA表达;采用Western blot法检测细胞膜GLUT_4蛋白表达。结果T_2~T_4时IR组血糖浓度明显高...

Objective To investigate the effect of desflurane post-processing on the expression of glucose transporter 4(GIUT_4)in myocardial ischemia-reperfusion injury.Methods Twenty-four male New Zealand white rabbits were randomly divided into 4groups(n=6each):normal control group(group NC),ischemic-reperfusion group (group IR),ischemic-reperfusion postconditioning group(group IRP),desflurane aftertreatment group(group Des).Myocardial ischemia-reperfusion model was established by ligating the left coronary artery.Plasma glucose,Insulin and myocardial glucose uptake rate were measured at the time point...