期刊论文

中文

生物化学与生物物理进展

1000-3282

2022

49

8

1530-1542

10.16476/j.pibb.2022.0231

National Natural Science Foundation of China [81500349]; Natural Science Foundation of Hunan Province, China [2020JJ4528, 2022JJ3059]; Key Program of Educational Commission of Hunan Province, China [21A0273]; Health and Family Planning Commission of Hunan Province, China [B2017048]

本校为第一机构

目的 本研究旨在阐明青蒿素对II型糖尿病（T2DM）小鼠认知功能障碍的改善作用及其机制。方法 C57BL/6J小鼠单次腹腔注射STZ （100 mg/kg）后联合高脂饲料喂养建立T2DM模型。T2DM小鼠随后腹腔注射青蒿素（40 mg/kg/d）或等体积溶剂。干预4周后，新物体识别、Y迷宫和Morris水迷宫实验检测小鼠的学习和记忆能力。蛋白质印迹法（Western blot）检测海马PI3K、Akt、磷酸化Akt、SYN和PSD-95蛋白的表达。透射电镜观察海马CA1区突触密度和突触超微结构改变。结果 与模型组相比，青蒿素干预组T2DM小鼠的认知功能显著改善，海马中PI3K和磷酸化Akt水平升高，SYN和PSD-95蛋白表达增加，CA1区神经元丢失减少。...

Objective The present study was to clarify the improving effect of artemisinin on diabetes-induced cognitive deficit and the underlying mechanisms in mice. Methods Type 2 diabetes mellitus (T2DM) mouse models were established by a single dose of STZ injection (100 mg/kg, i.p.). Those animals were then treated with vehicle or artemisinin (40 mg/kg, i.p.) once daily for 4 weeks. Cognitive performances of the mice were evaluated by novel object recognition, Y maze test and Morris water maze test. After behavioral tests, the expressions of PI3K, Akt, SYN and PSD-95 proteins in the hippocampus were...