Objective To investigated the function and the target gene of miR-216b in osteoclast differentiation and explored its effect on osteoclast cholesterol efflux.Methods The cell model of RAW264.7 osteoclast precursor cell differentiation induced by RANKL stimulation was established.Tartrate-resistant acid phosphatase(TRAP)staining assay was conducted to evaluate osteoclasts differentiation.MiR-216b target gene,ABCG1 3' untranslated region(3'UTR)sequence and free energy were predicted and analyzed by bioinformatics analyses and dual-luciferase reporter assays.MiR-216b mimic or inhibitor transfecti...
