期刊论文

DADS on The Biological Behavior of Human Leukemia HL-60 Cells With Nuclear Localization and Overexpression of DJ-1

Tan Hui

英文

生物化学与生物物理进展

1000-3282

2019

46

2

201-210

10.16476/j.pibb.2018.0194

Construct Program of the Key Discipline in Hunan Province of China [(2011) 76]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81100375, 81400117]; Platform Open Innovation Fund Project for Hunan Province Universities [12K094]

本校为第一且通讯机构

Recent studies have revealed that DJ-1 is overexpressed in multiple forms of human tumors, and nuclear overexpression of DJ-1 is correlated with the biological behavior of tumor. The purpose of the present study was to test for possible DADS effects on Human Leukemia HL-60 cells which DJ-1 is overexpressed, and to clarify the function of DJ-1 in the nucleus. Gene transfection technology was used to establish stable HL-60 cell lines with nuclear overexpression of DJ-1 (DJ-1/HL-60). Soft agar colony formation, MTT, NBT and indirect immunofluorescence were used to evaluate the proliferation, diff...

最近研究表明,DJ-1在许多肿瘤中过表达,而且DJ-1的核表达与肿瘤的生物学行为有关.本文主要研究二烯丙基二硫(DADS)对DJ-1核定位高表达人白血病HL-60细胞生物行为学的影响,阐明DJ-1在细胞核内的功能,为临床诊断及治疗过程提供一个潜在的治疗靶点.通过基因转染技术建立核内高表达HL-60细胞株(DJ-1/HL-60),利用软琼脂集落形成实验、 MTT法、间接免疫荧光细胞化学实验、硝基蓝四氮唑(NBT)还原比色实验评估HL-60细胞的增殖与分化,DJ-1核定位过表达促进HL-60细胞的增殖并抑制其分化. Transwell迁移侵袭小室实验表明DJ-1核定位过表达可以促进HL-60的迁移和侵袭能力. Western blot结果表明DADS具有抑制H...