期刊论文

Autophagy of Macrophages Induced by Oxidized Low-Density Lipoprotein via Wnt5a/PKCδ Signaling Pathway

Qin Li

中文

生物化学与生物物理进展

1000-3282

2019

46

6

596-602

10.16476/j.pibb.2019.0022

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81774130, 81670268]; Science Foundation for Distinguished Young Scholars of Hunan ProvinceNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [2018JJ1018]; Key Projects of Hunan Traditional Chinese Medicine Administration [201614]; Key Projects of Hunan Provincial Education Department [I5A141]; "13th Five-Year" First-level Discipline (Pharmacy) Funded Project of Hunan University of Chinese Medicine

本校为其他机构

研究发现动脉粥样硬化(atherosclerosis,AS)斑块中巨噬细胞摄取氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)和巨噬细胞极化等关键变化与失调性自噬关系密切.Wnt5a (wingless-type MMTV integration site family member 5a)在AS病变的富含巨噬细胞区域中高表达,然而Wnt5a 是否参与巨噬细胞自噬尚未明确.本研究发现,60mg/L ox-LDL处理Raw264.7细胞6h 时,自噬标志物LC3Ⅱ/Ⅰ显著增加,p62显著减少,且Wnt5a、PKCδ 及STAT3的表达均增加.小分子干扰RNA(small interference RNA,siRNA)敲低Wnt5a 后,逆转ox-LDL 诱导的LC3Ⅱ/Ⅰ和PKCδ 表达,上调p62表达,减少细胞内脂质蓄积.PKCδ 抑制剂...

Evidence indicated that key changes in macrophage uptake of oxidized low density lipoprotein (oxLDL) and macrophage polarization in atherosclerotic plaques are closely related to dysfunctional autophagy. Wnt5a (wingless-type MMTV integration site family member 5a) is highly expressed in the macrophage-rich region of atherosclerosis (AS) lesions. However, whether Wnt5a is involved in macrophages autophagy is not clear. In this study, we established macrophages-derived foam cell induced by ox-LDL to explore the effects of Wnt5a/PKC delta pathway on autophagy. RAW 264.7 macrophages were incubated...