期刊论文

Liao, DF

中文

生物化学与生物物理进展

1000-3282

2015

42

9

825-832

10.16476/j.pibb.2015.0111

National Natural Science Foundation of China [31371161, 81173047]; Natural Science Foundation of Hunan Province [2015JJ6077, 2014JJ1024]; Construct Program of the Pharmaceutical Science Key Discipline in Hunan Province; Innovation Team in University of South China

本校为第一机构

为研究PCSK9/LDLR通路介导姜黄素烟酸酯(CurTn)降低血浆低密度脂蛋白胆固醇(LDL-C),减少动脉内膜下脂质沉积的分子机制,用5、10、15 μmo/L姜黄素烟酸酯与25 mg/L LDL共孵育HepG2细胞24 h,分别采用油红O染色、胆固醇荧光定量试剂盒、DiI-LDL摄取检测细胞内胆固醇含量及LDL摄取情况,用逆转录定量聚合酶链反应(RT-Q-PCR)检测LDLR及SREBP2的mRNA表达,蛋白质印迹检测LDLR、SREBP2及PCSK9蛋白表达.随姜黄素烟酸酯作用浓度的增高细胞内脂滴显著增多,细胞内游离胆固醇(FC)、总胆固醇(TC)含量增高,细胞内胆固醇摄取增多;RT-Q-PCR和蛋白质印迹检测发现,与对照组(Control)比较,5、10、15 μmo/L姜黄素烟酸...

Low-density lipoprotein cholesterol (LDL-C) is a major risk factor for the atherosclerotic cardiovascular disease (ASCVD). Over 70% of circulating LDL-C is metabolized by binding hepatic LDL receptor (LDLR). Therefore, elevation of LDLR expression would reduce the progression of AS VCD. In order to study the molecular mechanism of Curcumin Nieotinate (CurTn) reducing lipid deposition of arterial intima by lowering plasma LDL-C, HepG2 cells were treated with 5, 10, 15 μmol/L CurTn co-incubated with 25 mg/L LDL for 24 h. Cellular lipid was detec...