期刊论文

Wang Zuo

中文

生物化学与生物物理进展

1000-3282

2013

40

8

757-765

10.3724/SP.J.1206.2012.00561

国家自然科学基金资助项目(81070221).This work was supported by a grant from The National Natural Science Foundation of China (81070221)

本校为第一且通讯机构

改善血流、促进血管新生是缺血性外周血管疾病的重要治疗措施.由于载脂蛋白A(ApoA)与纤溶酶原(plasminogen,Plg)具有75%~98%的结构同源性,因此,ApoA也可能通过类似Plg的方式抑制内皮祖细胞(endothelial progenitor cells,EPCs)增殖、黏附及迁移而影响血管发生的能力.本文研究ApoA对EPCs 血管发生的影响及机制.为了编码人ApoA全长cDNA序列的pSG-5表达载体,转染COS-7细胞株后进行培养,收集培养液,免疫亲和层析法分离纯化ApoA蛋白;从转ApoA基因小鼠、野生型对照鼠及正常对照鼠骨髓分离培养EPCs,经ApoA处理后移植下肢缺血实验小鼠,于移植后第3、7、14天后观察ApoA对EPCs黏附、迁移及血管发生能力的影...

Improvement of blood flow and promotion of neovascularization are important therapeutic measures for ischemic peripheral vascular diseases. Since apolipoproteinA(ApoA) is a glycoprotein with repetitive kringle domains exhibiting 75% to 98% structural homology with plasminogen(Plg), ApoA may also have a negative effect on endothelial progenitor cell (EPC)-induced vasculogenesis through Plg-like inhibitory effects on EPC proliferation, adhesion, migration and vasculogenesis. To evaluate the effect of ApoA on EPCs-induced vasculogenesis. ApoA was ...