期刊论文

Qin Xu-Ping

英文

生物化学与生物物理进展

1000-3282

2013

40

5

445-453

10.3724/SP.J.1206.2012.00028

grants from The National Natural Science Foundation of China(30572192,81173060);Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.国家自然科学基金资助项目(30572192,81173060);湖南省创新团队项目

本校为第一且通讯机构

Caveolae and caveolin-1 participate in the transportation of cholesterol and cell signal transduction. Our previous studies showed that the inhibitory effect of calcitonin gene-related peptide (CGRP) on the vascular smooth muscle cells (VSMCs) was related to decreasing the activity of extracellular signal-regulated kinase (ERK)_(1/2) and increasing the expression of caveolin-1. In the present study, we investigated the role of caveolae and caveolin-1 in proliferation of VSMCs and whether there are interaction between the caveolin-1 and ERK_(1/2...

业已证明,Caveolae及其蛋白caveolin-1参与了细胞膜的胆固醇转运和细胞膜的信号转导.我们前期工作发现降钙素基因相关肽（CGRP）抑制血管平滑肌细胞（VSMC）增殖的信号通路与抑制ERK1/2活性和上调caveolin-1表达有关.本文研究Caveolae及caveolin-1在CGRP抑制VSMC增殖中的作用,进一步研究caveolin-1表达增加是否有直接抑制ERK1/2信号激酶活性的作用.采用大鼠主动脉贴块法培养VSMC,取3～10代VSMC用于实验,10%小牛血清（FBS）用于刺激VSMC增殖,用β-环糊精（cyclodextrin）或菲律宾菌素（filipin）剥夺胆固醇破坏Caveolae结构;MTT法和流式细胞仪用于检测细胞增殖;蛋白质印迹和免疫共沉淀法分别用于...