The Effects of Highexpression and Knockdown Adipophilin in The Activity of ERK1/2 and Expression of PPAR_γ and Lipid Accumulation in Cells

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成果类型：

期刊论文

作者：

Liu Qing-Nan;Dai Zhi-Bing;Liu Zhi-Qiang;Tang Chao-Ke;Tian Guo-Ping;...

通讯作者：

Yuan Zhong-Hua

作者机构：

[Liu Zhi-Qiang; Tang Chao-Ke; Yuan Zhong-Hua; Liu Qing-Nan] Univ S China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.

[He Qiu; Ye Ling; Liu Qing-Nan; Dai Xiao-Song] YiYang Med Coll, Dept Basic Nursing, Yiyang 413000, Peoples R China.

[Dai Zhi-Bing] Cent Hosp Yiyang, Dept Type Ultrason Diag B, Yiyang 413000, Peoples R China.

[Tian Guo-Ping] Univ S China, Affiliated Hosp 2, Dept Cardiovasc Med, Hengyang 421001, Peoples R China.

通讯机构：

[Yuan Zhong-Hua] U

Univ S China, Key Lab Arteriosclerol Hunan Prov, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.

语种：

英文

关键词：

adipophilin;extracellular signal-regulated kinase1/2;peroxisome proliferator-activated receptor gamma;retroviruses

关键词(中文)：

细胞外信号调节激酶1/2;过氧化物酶体增殖物激活受体γ;逆转录病毒

期刊：

生物化学与生物物理进展

ISSN：

1000-3282

年：

2011

卷：

期：

页码：

1132-1144

DOI：

10.3724/SP.J.1206.2011.00112

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30971268]; Scientific Research Foundation for Returned Overseas Chinese Scholars from Ministry of EducationScientific Research Foundation for the Returned Overseas Chinese Scholars [(2008)890]; University of South China [5-XQD-2007-7]; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

机构署名：

本校为第一且通讯机构

院系归属：

医学院

摘要：

Our previous studies have showed that adipophilin promoted intracellular lipids accumulation through ERK1/2-PPAR gamma signaling pathway. In the study we explored that whether highexpression and knockdown adipophilin affects the activity of ERK1/2 and the expression of PPAR gamma and lipid accumulation in RAW264.7 cells, and further certified that adipophilin promoted intracellular lipids accumulation through this pathway. The recombinant retroviral vetors pQCXIP-HA-Adipophilin and pSuper-retro-adipophilin siRNA were verified by the methods of enzyme-digesting. The recombinant retroviral vetor...

摘要(中文)：

本课题组以前的研究表明,adipophilin通过ERK1/2-PPARγ信号转导通路促进细胞内的脂质蓄积.为了研究高表达和敲减adipophilin是否影响RAW264.7细胞内ERK1/2的活性、PPARγ的表达以及细胞内的脂质蓄积,从而进一步证实这一通路,阐明adipophilin促进泡沫细胞形成的机制.重组pQCXIP-HA-Adipophilin和pSuper-retro-adipophilin siRNA逆转录病毒载体经酶切检测证实,并用SofastTM介导转染到包装细胞PA317中,经培养后释放逆转录病毒.将收集的逆转录病毒感染RAW264.7细胞,经嘌呤霉素筛选后获得稳定高表达和敲减adipophilin的细胞系.用50 mg/L的氧化低密度脂蛋白处理细胞24 h后,用油红O染色法和高效液相色...

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The Effects of Highexpression and Knockdown Adipophilin in The Activity of ERK1/2 and Expression of PPAR_γ and Lipid Accumulation in Cells

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