期刊论文

He Xiu-Sheng

中文

生物化学与生物物理进展

1000-3282

2010

37

2

167-174

10.3724/SP.J.1206.2009.00492

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30470967]; National Natural Sciences Foundation of Hunan [03Jjy3029]; National Science Fund of China for Post-Doctor [2004035652]

本校为第一且通讯机构

为研究鼻咽癌相关新基因NPCEDRG的功能,探讨其对鼻咽癌细胞生长特性的影响,利用Tet-on调控系统,建立受强力霉素(deoxycycline,Dox)诱导NPCEDRG基因表达的CNE2细胞系.运用RT-PCR选择背景表达低、诱导活性高的细胞克隆,以不同浓度Dox诱导CNE2/Tet/TRE-NPCEDRG细胞,确定Dox的最佳诱导浓度.借助形态学观察、细胞生长曲线、软琼脂克隆形成试验和流式细胞仪分析等方法,对Dox诱导NPCEDRG高表达后CNE2细胞的生物学行为进行了检测.结果显示,NPCEDRG高表达后CNE2细胞增殖速度显著减慢(P

To investigate the inhibitory function of NPCEDRG, a novel tumor suppressor gene, in nasopharyngeal carcinoma (NPC), Tet-on system was introduced. Two plasmids were co-transfected into CNE2 cells, then a transgene CNE2 cell line, which had rapidly inducible and reversible expression of NPCEDRG, was obtained after two selections with G418 or hygromycin. NPCEDRG mRNA expression was detected via RT-PCR assay. Dox was used to induce the expression of NPCEDRG and a cell clone sensitive to Dox was selected. The best-induced concentration was determined with different concentration of Dox induction. ...