期刊论文

Ou He-Sheng

中文

生物化学与生物物理进展

1000-3282

2010

37

7

747-753

10.3724/SP.J.1206.2009.00755

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30670834, 30871186]; Science Foundation of Hunan Universities [06A060]

本校为第一且通讯机构

前期工作表明,内皮型一氧化氮合酶(endothelial nitric oxide synthase, eNOS)第4内含子中的27碱基(nucleotide, nt)重复序列是27-nt microRNA的来源,并对eNOS具有重要的调节作用.为进一步探讨该内含子源性27-nt microRNA参与调节eNOS表达的分子机制及其在内皮细胞增殖中的可能作用,通过构建27-nt microRNA高表达质粒,用脂质体将该质粒转染人脐静脉内皮细胞(human umbilical vein endothelial cells, HUVEC), Western blot和RT-PCR检测该细胞系中eNOS蛋白和 mRNA表达情况以及胞核转录因子的表达改变,并观察HUVEC增殖的变化情况.结果发现: 27-nt microRNA高表达能降低 eNOSmRNA的水平和蛋白质表达...

Previously it was reported that the 27-nucleotide(nt) repeats in intron 4 of endothelial nitric syanthase (eNOS) were the source of 27-nt microRNA, which play an important role in regulation of eNOS expression. In order to further study the molecular mechanisms in regulation of eNOS gene expression and endothelial cell proliferation by the 27-nt microRNA, it was constructed the 27-nt microRNA highly expression plasmid, which was transferred into HUVEC with lipofectamine. The level of eNOS protein and mRNA, as well as Sp1 and Ap-1, were measured...