期刊论文

Tang Chao-Ke

中文

生物化学与生物物理进展

1000-3282

2010

37

5

540-548

10.3724/SP.J.1206.2009.00738

National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30470720]

本校为第一且通讯机构

脂多糖(LPS)介导的免疫炎症反应与动脉粥样硬化(As)的发生密切相关, ATP结合盒转运体A1(ABCA1)促进细胞内胆固醇流出, 具有抗As作用.观察了LPS对THP-1巨噬细胞源性泡沫细胞ABCA1表达及胆固醇流出的影响, 并探讨TLR4/NF-κB信号途径和LXRs在此过程中的作用.THP-1巨噬细胞源性泡沫细胞经不同浓度LPS处理或者用LPS作用不同时间, 以LPS单独或用NF-κB抑制剂对甲苯磺酰-L-苯丙氨酸氯甲基甲酮(TPCK)预处理细胞后再加入LPS处理.RT-PCR检测 ABCA1、TLR4和LXRαmRNA的表达, Western blot检测ABCA1、LXRα及核内NF-κB p65蛋白的表达, 液体闪烁计数器检测细胞内胆固醇流出, 高效液相色谱分析细胞内总胆固醇...

The immnue-inflammatory response mediated by LPS is firmly related to the development of atherosclerosis. ABCA1 has been identified to play a key role in the cellular cholesterol efflux which is regarded to anti-atherosclerosis. To investigate the changes of cholesterol efflux, ATP-binding cassette transporter A1 (ABCA1) mRNA and protein expression in THP-1 macrophage derived foam cells treated with LPS, and to discover the role of TLR4/NF-kappa B pathway and LXRs in this process. The foam cells were exposed to different concentration of LPS for 24 h or exposed to LPS for different times, and ...