期刊论文

Tang Sheng-Song

中文

生物化学与生物物理进展

1000-3282

2009

36

10

1299-1305

10.3724/SP.J.1206.2009.00149

Hunan Provincial Natural Science Foundation of China [08115004, 09JJ3060]; The Key Scientific Research Project of Hunan Province [06FJ3203]; The Key Scientific Research Project of Department of Hunan Education [07A059]

本校为第一且通讯机构

二烯丙基三硫(diallyl trisulfide,DATS)对多种肿瘤有抗癌作用,但机制尚不完全清楚.为探讨DATS对人肝癌细胞系HepG2细胞凋亡的影响,用丫啶橙/溴化乙锭(AO/EB)法观察细胞凋亡情况,在显微镜下计数凋亡细胞数.JC-1荧光染色观察线粒体膜电势变化.Western blot法检测细胞色素c蛋白分布,ELISA法检测caspase-3活性.结果显示,DATS诱导HepG2细胞凋亡,用50 μmol/L与100 μmol/L DATS处理48 h,细胞凋亡率分别达到60.33%和93.67%,并引起HepG2 细胞线粒体膜电势降低.Western blot显示,DATS能诱导胞浆细胞色素c增加,与此同时,线粒体细胞色素c减少,诱导HepG2细胞caspas-3活化.提示:DATS可通过降低线粒体膜电势...

Diallyl trisulfide (DATS) is known to have an anticancer effect on human cancer cells. However, the exact mechanisms of this anticancer activity remain unclear. To explore the effect of DATS on human hepatocellular carcinoma HepG2 cell apoptosis. HepG2 cells were either treated with 50 mu mol/L, 100 mu mol/L of DATS for 0, 6, 12, 24, 48 and 72 h, respectively. The mitochondrial membrane potential was visualized by the JC-1 fluorescence staining. The cytochrome c in HepG2 cells was detected by Western blotting. The activity of caspase-3 in HepG2 cells was measured with ELISA. Diallyl trisulfide...