期刊论文

Liu Lu-Shan

中文

生物化学与生物物理进展

1000-3282

2009

36

3

323-330

10.3724/SP.J.1206.2008.00427

The National Natural Science Foundation of China [30700325]; Key Project of Science and Technology Department of Hunan Province [2008FJ2006]

本校为第一且通讯机构

为研究pcsk9基因沉默后对氧化型低密度脂蛋(oxLDL)诱导THP-1源性巨噬细胞凋亡的影响,用不同浓度oxLDL处理THP-1源性巨噬细胞48 h,Hoechst33258染色检测细胞凋亡,RT-PCR、Western blot分别检测pcsk9 mRNA、NARC-1蛋白的表达.应用Lipofectamine2000转染3对pcsk9 siRNAs进THP-1源性巨噬细胞中,筛选出最有效的siRNA再转染入THP-1源性巨噬细胞,24 h后加入oxLDL处理48 h,Hoechst33258染色观察细胞评价细胞凋亡,流式细胞术计数检测细胞凋亡率.结果发现,75 mg/L oxLDL处理THP-1源性巨噬细胞48 h后,Hoechst33258染色可见大量凋亡细胞.同时RT-PCR、Western blot检测发现,pcsk9 mRNA和NARC-1蛋白质表达量均...

In order to study the effect of pcsk9 siRNA on THP-1 derived macrophages apoptosis induced by oxLDL, THP-1 derived macrophages were induced to differentiate into macrophages by PMA treatment for 24 h. The experiments were designed as follows: cells were incubated with oxLDL with a concentration of 0, 25, 50, 75, 100 mg/L for 48 h respectively. The apoptosis of THP-1 derived macrophages was observed by staining with Hoechst33258. The expression of pcsk9 was analyzed by reverse transcription polymerase chain reaction and Western blot. The siRNAs for pcsk9 gene were designed and synthesized, then...