期刊论文

Chen Lin-Xi

中文

生物化学与生物物理进展

1000-3282

2009

36

12

1578-1588

10.3724/SP.J.1206.2009.00360

National Natural Science Foundation of China [30901577]; Outstanding Young People Education Fund Project of Hunan Province [03B036]

本校为第一且通讯机构

研究白发性高血压大鼠(spontanously hypertensive rat,SHR)离体血管环对G蛋白偶联受体APJ的内源性配体apelin-13的血管收缩与舒张反应及其与一氧化氮NO)和ERK1/2通路关系.采用离体血管环体外灌流方法用Power-Lab生物信息采集仪检测血管环的张力.实验分组如下:新福林(Phenylephrine,PE)组,乙酰胆碱(acetylcholine,Ach)组,apelin-13组,apelin-13+PE组,apelin-13+Ach组,PD98059(ERK1/2抑制剂)+PE组,PD98059+Ach组,LNNA(L-nitro-arginine,硝基左旋精氨酸,一氧化氮合酶抑制剂)+PE组,LNNA+Ach组,apelin-13(预孵育)+PD98059+PE组,apelin-13(预孵育)+PD98059+Ach组,apelin-13(预孵育)+LNNA+PE组和apeli...

In order to study the effect of G protein-coupled receptor APJ endogenous ligand apelin-13 to the vasoconstriction and relaxation of spontaneously hypertensive rat's vascular rings in vitro and its NO and ERK1/2 pathway, perfusion method in vitro vascular ring and Power-Lab system was used to detect tension on rat's vascular rings. Experimental groups below: Phenylephrine group, Acetylcholine group, apelin-13 group, apelin-13+ PE group, apelin-13+Ach group, PD98059+PE group, PD98059+Ach group, LNNA+PE group, LNNA+Ach group, apelin-13 (preincubation) +PD98059 +PE group, apelin (preincubation)-1...