期刊论文

Wang Zuo

中文

生物化学与生物物理进展

1000-3282

2008

35

7

807-813

10.3321/j.issn:1000-3282.2008.07.012

2005038472:中国博士后科学基金 07JJ3034:湖南省自然科学基金 07C617:湖南省教育厅项目

本校为第一且通讯机构

探讨AMD3100对apoE~(-/-)小鼠骨髓内皮祖细胞的动员作用及其增殖、迁移和黏附的影响.12只8周龄雄性apoE~(-/-)小鼠随机分为AMD3100组(2.5mg/(kg·2d))和对照组(PBS0.1ml/2d),高脂高胆固醇饲料喂养12周后,差速贴壁法结合微孔法分离培养小鼠骨髓细胞,免疫荧光鉴定CD133/VEGFR-2双阳性细胞为内皮祖细胞;MTT比色法、Transwell、黏附试验分别检测细胞的增殖、迁移和黏附能力;通过计数典型内皮祖细胞克隆形成单位,观察次级集落单位的大小及细胞密度,检测各组内皮祖细胞的克隆形成能力;RT-PCR和Westernblot检测内皮祖细胞上CXCR4mRNA和蛋白质表达水平.与对照组比较,AMD3100组骨髓源性内皮祖细胞的增殖、...

To study the effect of AMD3100 on the mobilization, proliferation, migration and adhesion of endothelial progenitor cells (EPC), EPC was isolated from apoE(-/-) mice bone marrow, 12 male apoE(-/-) mice, with 8 weeks old,were randomly divided into two groups, AMD3100 group (2.5mg / (kg center dot 2d)) and control group(PBS, 0.1 ml/2d). After feeding western (high fat and cholesterol) for 12 weeks, the bone marrow cells were isolated and cultured by way of differential-speed-adherence and Micropore-Method. CD133(+) VEGFR-2(+) bone marrow cell was identified as endothelial progenitor cells by imm...