期刊论文

Yang, YZ

中文

生物化学与生物物理进展

1000-3282

2004

31

6

543-549

10.3321/j.issn:1000-3282.2004.06.011

国家自然科学基金资助项目（3 0 3 70 675 ）； 湖南省科技厅重点资助项目（0 1SSY10 0 3 ）； 湖南省教育厅资助项目（0 2C3 99）~~；

本校为第一且通讯机构

用贵州小香猪建立2型糖尿病动物模型,探讨糖尿病小型猪三磷酸腺苷结合盒转运体A1(ABCA1)表达的变化.采用高脂高蔗糖饲料喂养贵州小香猪,建立2型糖尿病动物模型.血浆总胆固醇、甘油三酯、高密度脂蛋白胆固醇和葡萄糖的浓度均用氧化酶法测定,血浆游离脂肪酸(FFA)用比色法测定,采用逆转录-聚合酶链反应、蛋白质印迹和免疫组织化学分别检测ABCA1mRNA和蛋白质的表达.喂养6个月后,实验组与正常对照组比较,空腹血糖值明显升高;空腹胰岛素水平在头3个月轻度升高,在第6个月末其水平降低;血清总胆固醇、甘油三酯和游离脂肪酸水平升高;肝组织、冠状动脉、肾组织ABCA1表达上调,同时观测到糖尿病小型猪肝组织...

To establish a new animal model of the human type 2 diabetes mellitus and investigate the change of ATP binding cassette transporter A1 expression in diabetic minipigs, Chinese minipigs were fed a normal control diet ( CD) or a high fat/high sucrose diet (HFSD) for 6 months. Plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C, triglycerides (TG) and glucose were determined by commercially enzymatic methods. Plasma free fatty acids (FFA) were measured by a colorimetric method. ABCA1 mRNA and protein level were determined by reverse trancriptase-polymerase chaim reaction (...